An integrated microfluidic system for early detection of sepsis-inducing bacteria. Issue 1 (24th November 2020)

• Record Type: Journal Article
• Authors: Fang, Yen-Ling; Wang, Chih-Hung; Chen, Yi-Sin; Chien, Chun-Chih; Kuo, Feng-Chih; You, Huey-Ling; Lee, Mel S.; Lee, Gwo-Bin
• Is Part Of: Lab on a chip. Volume 21:Issue 1(2021)
• Page Start: 113
• Page End: 121
• Publication Date: 2020-11-24
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/d0lc00966k
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : Isolation and identification of bacteria from human blood were automated on an integrated microfluidic system for sepsis-inducing bacteria. Abstract : Since early diagnosis of sepsis may assist clinicians in initiating timely, effective, and prognosis-improving antibiotic therapy, we developed an integrated microfluidic chip (IMC) for rapid isolation of both Gram-positive and Gram-negative bacteria from blood. The device comprised a membrane-based filtration module (90 min operating time), a bacteria-capturing module using a micro-mixer containing magnetic beads coated with "flexible neck" regions of mannose-binding lectin proteins for bacteria capture (20 min), and a miniature polymerase chain reaction (PCR) module for bacteria identification (90 min via TaqMan® probe technology). The filter separated all white blood cells and 99.5% of red blood cells from bacteria, which were captured at rates approaching 85%. The PCR assay's limit of detection was 5 colony-forming units (CFU) per reaction, and the entire process was completed in only 4 h. Since this is far less than that for culture-based approaches, this IMC may serve as a promising device for detection of sepsis.