An azo dye for photodynamic therapy that is activated selectively by two-photon excitation. Issue 1 (6th November 2020)

• Record Type: Journal Article
• Authors: Juvekar, Vinayak; Lim, Chang Su; Lee, Dong Joon; Park, Sang Jun; Song, Gyeong Ok; Kang, Hyuk; Kim, Hwan Myung
• Is Part Of: Chemical science. Volume 12:Issue 1(2021)
• Page Start: 427
• Page End: 434
• Publication Date: 2020-11-06
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0sc05686c
• Languages: English
• ISSNs: 2041-6520

Abstract

Abstract : Azo containing dyes as a two-photon selective and type I based photosensitizers (PSs) were developed that exhibit excellent photodynamic therapy properties under mild condition. Abstract : Two-photon photodynamic therapy (TP-PDT) is a promising approach for the treatment of cancer because of its better penetration depth and superior spatial selectivity. Here, we describe an azo group containing cyclized-cyanine derivatives (ACC1 and ACC2 ) as a two-photon activated, type I based photosensitizer (PS). These small-molecule and heavy atom-free organic dyes showed marked reactive oxygen species (ROS)-generating ability under physiological conditions, as well as fast loading ability into the cells and negligible dark toxicity. Live cell analyses with one- and two-photon microscopy revealed that these dyes showed higher ROS generation ability upon two-photon excitation than upon one-photon excitation via the type I process. The PSs have superior PDT properties compared to conventional Visudyne and 5-ALA under mild conditions. These characteristics allowed for precise PDT at the target region in mimic tumor spheroids, demonstrating that the developed TP PS could be useful in efficient PDT applications and in designing various PSs.