Green facile synthesis to develop nanoscale coordination polymers as lysosome-targetable luminescent bioprobes. (27th October 2020)

• Record Type: Journal Article
• Authors: Karim, Suhana; Mukherjee, Somali; Mahapatra, Supratim; Parveen, Rumana; Das, Debasis
• Is Part Of: Biomaterials science. Volume 9:Number 1(2021)
• Page Start: 124
• Page End: 132
• Publication Date: 2020-10-27
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/d0bm01328e
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : A facile green hand grinding technique has been adopted towards the design of three nanoscale crystalline materials that serves as an excellent selective bio tracker for intracellular lysosome. Abstract : Three new coordination polymers (CP s), namely [{M(HL)(L)(H2 O)}(ClO4 )(H2 O)]∞ (M = Zn for CP 1, Mn for CP 2, Cu for CP 3 ) were synthesized to explore their efficacy as lysosome-targetable luminescent bioprobes. The synthesized CP s were characterized by techniques including single-crystal X-ray analysis, FTIR spectroscopy and elemental analysis. Single-crystal analysis revealed the formation of iso-structural CP s displaying distorted adamantoid topology developed by bridging ligands and H-bonds connections and metals at the nodes. A green hand-grinding technique with a mortar and pestle resulted in nanoscale coordination polymers (NCP s) suitable for cell permeability and was further confirmed by SEM and DLS analyses. Two of these hand-ground nanoscale coordination polymers NCP 1 and NCP 2 showed excellent green luminescence and were explored as potential and selective long-time biotrackers towards lysosome using the human lung carcinoma cell line (A549). Strikingly, the developed bioprobe displayed excellent bio-availability, photostability and excellent selectivity towards lysosomes sustained by various in vitro cell imaging experiments. Moreover, the long-term probing ability of these NCP s turned out to be better than the commercially available lysosome tracker i.e. LysoTracker Red, indicating their potential real-life application in bio-imaging. To the best ofour knowledge, this is the first example of nonexpensive and less toxic essential transition metal-based nanoscale coordination polymers that can behave as effective lysosome-targetable luminescent bioprobes.