Cascade CRISPR/cas enables amplification-free microRNA sensing with fM-sensitivity and single-base-specificity. Issue 2 (11th December 2020)

• Record Type: Journal Article
• Authors: Sha, Yong; Huang, Ru; Huang, Mengqi; Yue, Huahua; Shan, Yuanyue; Hu, Jiaming; Xing, Da
• Is Part Of: Chemical communications. Volume 57:Issue 2(2021)
• Page Start: 247
• Page End: 250
• Publication Date: 2020-12-11
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc06412b
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A cascade CRISPR/cas nucleic acid diagnostic system, which can achieve high-sensitive and single-base specificity without target amplification, was developed for miRNA detection. Abstract : Existing CRISPR/cas-based biosensors usually improve sensitivity by target amplification, which is time-consuming and susceptible to impurities in complex biofluid. Herein, this is the first time a cascade CRISPR/cas (casCRISPR) system has been developed, which can provide a detection limit of 1.33 fM (∼1000 times lower than direct Cas13a-based miRNA detection) and single-base resolution for miR-17 detection without resorting to target amplification. casCRISPR can also be applied to detect miRNA in complicated cell extracts and serum samples. Overall, casCRISPR will provide a heuristic idea for CRISPR/cas based biosensing, and could be a promising tool for miRNA diagnostics.