Colorimetric detection of ciprofloxacin in aqueous solution based on an unmodified aptamer and the aggregation of gold nanoparticles. Issue 1 (10th December 2020)

• Record Type: Journal Article
• Authors: Huang, Weiwen; Wang, Yichen; Wang, Lumei; Pan, Chaoqiang; Shen, Guoqing
• Is Part Of: Analytical methods. Volume 13:Issue 1(2021)
• Page Start: 90
• Page End: 98
• Publication Date: 2020-12-10
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d0ay01811b
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Sensitive and selective colorimetric detection of ciprofloxacin based on an unmodified aptamer and the aggregation of AuNPs induced by PDDA. Abstract : A colorimetric method is described for detection of the antibiotic ciprofloxacin (CIP) in aqueous solutions based on an unmodified CIP-aptamer and gold nanoparticles (AuNPs), which are regarded as a probe and an indicator, respectively. In the absence of CIP, aptamers can hybridize with poly dimethyl diallyl ammonium chloride (PDDA) to form a 'duplex' structure through electrostatic interactions, and AuNPs remain dispersed in solution, leading to a red solution and an obvious absorption peak at 520 nm. Contrarily, the CIP-aptamer can specifically bind to CIP after the introduction of CIP into the solution. Then PDDA is thus free to aggregate AuNPs. The solution turns blue from red accordingly, presenting a strong absorption at 650 nm. Hence, the concentration of CIP can be quantified through the changes of the absorption. This sensitive and selective colorimetric method for CIP detection has a good linear response ( R = 0.9935) in the 20 to 300 nM CIP concentration range, with a limit of detection (LOD) of 0.215 nM. Importantly, the proposed aptasensor demonstrates great application potential in CIP determination in aqueous samples.