A novel ruthenium(ii) gallic acid complex disrupts the actin cytoskeleton and inhibits migration, invasion and adhesion of triple negative breast tumor cells. Issue 1 (11th December 2020)

• Record Type: Journal Article
• Authors: Graminha, Angelica E.; Honorato, João; Correa, Rodrigo S.; Cominetti, Marcia R.; Menezes, Antônio C. S.; Batista, Alzir A.
• Is Part Of: Dalton transactions. Volume 50:Issue 1(2020)
• Page Start: 323
• Page End: 335
• Publication Date: 2020-12-11
• Subjects: Chemistry, Inorganic -- Periodicals; Chemistry, Physical and theoretical -- Periodicals; Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/d0dt03490h
• Languages: English
• ISSNs: 1477-9226

Abstract

Abstract : Ru(ii ) complexes with gallic acid and derivatives interact with biomolecules and show high SI among non-tumor/tumor breast cells. The gallic acid complex causes damage to the cell cytoskeleton inhibiting adhesion, invasion, and migration of tumor cells. Abstract : This work describes the synthesis of three new ruthenium(ii ) complexes with gallic acid and derivatives of the general formula [Ru(L)(dppb)(bipy)]PF6, where L = gallate (GAC), benzoate (BAC), and esterified-gallate (EGA), bipy = 2, 2′-bipyridine and dppb = 1, 4-bis(diphenylphosphino)butane. The complexes were characterized by elemental analysis, molar conductivity, NMR, cyclic voltammetry, UV-vis and IR spectroscopy, and two of them by X-ray crystallography. Cell viability assays show promising results, indicating higher cytotoxicity of the complexes in MDA-MB-231 cells, a triple-negative breast cancer (TNBC) cell line, compared with the hormone-dependent MCF-7 cell line. Studies in vitro with the MDA-MB-231 cell line showed that only Ru(BAC) and Ru(GAC) interacted with BSA. Besides that, the Ru(GAC) complex, which has a polyphenolic acid, interacted in an apo-Tf structure and function dependent manner and it was able to inhibit the formation of reactive oxygen species. Ru(GAC) was able to cause damage to the cellular cytoskeleton leading to inhibition of some cellular processes of TNBC cells, such as invasion, migration, and adhesion.