Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs. Issue 4 (25th October 2014)

• Record Type: Journal Article
• Authors: Wang, Huili; Yan, Xin; Shen, Liangyun; Li, Shiyan; Lin, Yue; Wang, Shuqin; Hou, Xiang Lin; Shi, Chunying; Yang, Yun; Dai, Jianwu; Tan, Qian
• Is Part Of: Burns and trauma. Volume 2:Issue 4(2014)
• Publication Date: 2014-10-25
• Subjects: Mesenchymal stem cells -- skin wound healing -- Collagen-binding domain -- mesenchymal stem cells affinity peptide; Burns and scalds -- Periodicals; Wounds and injuries -- Periodicals; 617.11
• DOI: 10.4103/2321-3868.143623
• Languages: English
• ISSNs: 2321-3876

Abstract

Abstract: Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. In vivo investigations indicated that due to the immunore-action, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups ( P < 0.05, P < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds.