Ecofriendly conversion of algal waste into valuable plant growth-promoting rhizobacteria (PGPR) biomass. (1st February 2021)

• Record Type: Journal Article
• Authors: Yuan, Yuan; Chu, Depeng; Fan, Jiajun; Zou, Ping; Qin, Yimin; Geng, Yuting; Cui, Zhenzhen; Wang, Xiaohui; Zhang, Chengsheng; Li, Xiangdong; Clark, James; Li, Yiqiang; Wang, Xiaoqiang
• Is Part Of: Waste management. Volume 120(2021)
• Page Start: 576
• Page End: 584
• Publication Date: 2021-02-01
• Subjects: Algal waste -- PGPR -- Microwave -- Cultivation -- Pepper; PGPR plant growth-promoting rhizobacteria -- LJW Laminaria japonica processing waste -- EP Enteromorpha prolifera -- IAA indole-3-acetic acid -- LB Luria-Bertani -- TFA trifluoroacetic acid -- NTC None treatment control -- Man mannose -- Rha rhamnose -- GlcA glucuronic acid -- GalA galacturonic acid -- Glc glucose -- Gal galactose -- Xyl xylose -- Fuc fucose; Hazardous wastes -- Periodicals; Refuse and refuse disposal -- Periodicals; 363.728
• DOI: 10.1016/j.wasman.2020.10.020
• Languages: English
• ISSNs: 0956-053X

Abstract

Graphical abstract: Highlights: Up to 50% (w/w%) hydrolysate yield was obtained from waste algal biomass. Microwave-assisted hydrolysis process is a practical approach. PGPR strains were successfully cultivated in hydrolysate without extra nutrients. Pepper growth was significantly promoted by hydrolysate-PGPR cultivation broth. Abstract: With the development of marine biorefinery concept, utilisation of algal waste during industrial processing as well as some "green tide" waste biomass has become an important research topic. In this work, a single-step microwave process was used to hydrolyse Laminaria japonica processing waste (LJW) and Enteromorpha prolifera (EP), producing a growth medium suitable for microbial cultivation. The medium contained a range of mono- and polysaccharides as well as macro- and micronutrients that could be used by the microbes. The cultivation behavior of three plant growth-promoting rhizobacteria (PGPR) strains ( Bacillus subtilis strain Tpb55, Bacillus amyloliquefaciens strain Cas02, and Burkholderia pyrrocinia strain Lyc2) in the two media were investigated. LJW hydrolysate from 180 °C and EP hydrolysate from 150 °C performed better cultivation efficiency than those hydrolysates from other microwave conditions. Saccharide analysis showed that microbes metabolized some monosaccharide such as glucose, mannose during cultivation, leaving polysaccharide unused in the medium. Furthermore, hydrolysate-strain cultivation mixtures were applied to pepper growth. The EP hydrolysate-Cas02 broth showed better plant growth-promoting effect compared to other treatments, which might be attributed to the higher indole-3-acetic acid (IAA) production of Cas02 in the EP hydrolysate. This work shed lights on the conversion of algal waste to PGPR biomass as well as the co-application of algal hydrolysates- strains cultivation broth for a better plant growth promotion.