3D finite element models from serial section histology of skeletal muscle tissue – The role of micro-architecture on mechanical behaviour. (January 2021)

• Record Type: Journal Article
• Authors: Kuravi, R.; Leichsenring, K.; Böl, M.; Ehret, A.E.
• Is Part Of: Journal of the mechanical behavior of biomedical materials. Volume 113(2021)
• Publication Date: 2021-01
• Subjects: Skeletal muscle -- Mechanical behaviour -- Histology -- Image analysis -- Extracellular matrix -- Microstructural modelling; Biomedical materials -- Periodicals; Biomedical materials -- Mechanical properties -- Periodicals; Biomedical materials; Biomedical materials -- Mechanical properties; Periodicals; Electronic journals; 610.28
• DOI: 10.1016/j.jmbbm.2020.104109
• Languages: English
• ISSNs: 1751-6161

Abstract

Abstract: In this contribution we create three-dimensional (3D) finite element models from a series of histological sections of porcine skeletal muscle tissue. Image registration is performed on the stained sections by affinely aligning them using auxiliary markers, followed by image segmentation to determine muscle fibres and the extracellular matrix in each section, with particular regard to the continuity of the fibres through the stack. With this information, 3D virtual tissue samples are reconstructed, discretised, and associated with appropriate non-linear elastic anisotropic material models. While the gross anatomy is directly obtained from the images, the local directions of anisotropy were determined by the use of an analogy with steady state diffusion. The influence of the number of histological sections considered for reconstruction on the numerically simulated mechanical response of the virtual tissue samples is then studied. The results show that muscle tissue is fairly heterogeneous along the fascicles, and that transverse isotropy is inadequate in describing their material symmetry at the typical length scale of a fascicle. Numerical simulations of different load cases suggest that ignoring the undulations of fibres and their non-uniform cross-sections only moderately affects the passive response of the tissue in tensile and compressive modes, but can become crucial when predicting the response to generic loads and activation. Graphical abstract: Highlights: 3D finite element models of skeletal muscle tissue are developed from serial histological sections. The effect of 3D structure on mechanical behaviour is studied in virtual experiments. The 3D microstructure leads to heterogeneity and complex material symmetry. The effect of ignoring the complex 3D structure in models can be very strong, but depends on the load case.