A conserved double‐W box in the promoter of CaWRKY40 mediates autoregulation during response to pathogen attack and heat stress in pepper. (5th November 2020)

• Record Type: Journal Article
• Authors: Liu, Zhi‐Qin; Shi, Lan‐Ping; Yang, Sheng; Qiu, Shan‐Shan; Ma, Xiao‐Ling; Cai, Jin‐Sen; Guan, De‐Yi; Wang, Zong‐Hua; He, Shui‐Lin
• Is Part Of: Molecular plant pathology. Volume 22:Number 1(2021)
• Page Start: 3
• Page End: 18
• Publication Date: 2020-11-05
• Subjects: double‐W box‐element -- pepper -- promoter -- Ralstonia solanacearum -- transcription factor -- WRKY; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.13004
• Languages: English
• ISSNs: 1464-6722

Abstract

Abstract: CaWRKY40 was previously found to be transcriptionally up‐regulated by Ralstonia solanacearum inoculation (RSI) or heat stress (HS), but the underlying mechanism remains unknown. Herein, we report that a double‐W box‐element (DWE) in the promoter of CaWRKY40 is critical for these responses. The upstream W box unit WI of this composite element is crucial for preferential binding by Ca WRKY40 and responsiveness to RSI or HS. DWE‐driven CaWRKY40 can be transcriptionally and nonspecifically regulated by itself and by Ca WRKY58 and Ca WRKY27. The DWE was also found in the promoters of CaWRKY40 orthologs, including AtWRKY40, VvWRKY40, GmWRKY40, CplWRKY40, SaWRKY40, SpWRKY40, NtWRKY40, and NaWRKY40 . DWE AtWRKY40 was analogous to DWE CaWRKY40 by responding to RSI or HS and At WRKY40 expression. These data suggest that a conserved response of plants to pathogen infection or HS is probably mediated by binding of the DWE by WRKY40. Abstract : Our study reports that a conserved response of plants to pathogen infection or heat shock is probably mediated by the binding of the double‐W box to WRKY40.