Microbiota profile and efficacy of probiotic supplementation on laxation in adults affected by Prader‐Willi Syndrome: A randomized, double‐blind, crossover trial. Issue 12 (25th October 2020)

• Record Type: Journal Article
• Authors: Alyousif, Zainab; Miller, Jennifer L.; Auger, Jeremie; Sandoval, Mariana; Piano, Amanda; Tompkins, Thomas A.; Dahl, Wendy J.
• Is Part Of: Molecular genetics & genomic medicine. Volume 8:Issue 12(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-10-25
• Subjects: Bristol Stool Form -- constipation -- HEI‐2015 -- Prader‐Willi syndrome -- probiotic -- stool frequency; Medical genetics -- Periodicals; Genomics -- Periodicals; 616.042
• DOI: 10.1002/mgg3.1535
• Languages: English
• ISSNs: 2324-9269

Abstract

Abstract: Background: Probiotics may provide a benefit for adults with Prader‐Willi syndrome (PWS) experiencing constipation. The primary aim was to determine if Bifidobacterium animalis ssp. lactis B94 ( B . lactis B94) improves stool frequency, with secondary aims of stool form and gastrointestinal symptoms. Exploratory aims included diet quality and fecal microbiota composition. Methods: Following a 4‐week baseline, 25 adults with PWS were randomized to consume B . lactis B94 by capsule (15 billion) or placebo for 4 weeks, followed by 4‐week washout in a double‐blind, crossover design. Stool frequency and Bristol Stool Form (BSF) were assessed daily, and Gastrointestinal Symptom Rating Scale (GSRS) and dietary intake (7‐days food records), per period. Fecal microbiota per period was analyzed using 16S rRNA gene amplicon sequencing and taxa of interest by qPCR ( n = 24). Results: No adverse events were reported. Stool frequency at baseline ( n = 25; 2.0 ± 0.1 stools/day), GSRS syndromes, and microbiota composition did not differ with the probiotic intervention overall; however, a delayed, carry‐over effect on BSF types 6 and 7 was seen. Diet quality by HEI‐2015 was 65.4 ± 8.5. Conclusion: In adults with PWS, B . lactis B94 exhibited little effect on laxation over 4 weeks; however, further research is needed. Abstract : In adults affected by Prader‐Willi syndrome, and consuming an energy‐controlled diet of moderate diet quality, administration of the probiotic, Bifidobacterium animalis ssp. lactis B94, does not impact microbiota profiles.