Design of experiments driven optimization of alkaline pretreatment and saccharification for sugarcane bagasse. (February 2021)

• Record Type: Journal Article
• Authors: Mota, Thatiane R.; Oliveira, Dyoni M.; Simister, Rachael; Whitehead, Caragh; Lanot, Alexandra; dos Santos, Wanderley D.; Rezende, Camila A.; McQueen-Mason, Simon J.; Gomez, Leonardo D.
• Is Part Of: Bioresource technology. Volume 321(2021)
• Publication Date: 2021-02
• Subjects: Biomass pretreatment -- Central composite orthogonal design -- Fractional factorial design -- Lignocellulose -- Alkaline pretreatment; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124499
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Simultaneous optimisation of pretreatment and saccharification using design tool. High-resolution Design of Experiments maximizes sugar release in sugarcane bagasse. Fractional Factorial Design was combined with a Central Composite Orthogonal design. Design of Experiments monitored by compositional analysis of biomass. Abstract: To maximize the sugar release from sugarcane bagasse, a high-resolution Fractional Factorial Design (FFD) was combined with a Central Composite Orthogonal (CCO) design to simultaneously evaluate a wide range of variables for alkaline pretreatment (NaOH: 0.1–1 mol/L, temperature: 100–220 °C, and time: 20–80 min) and enzymatic saccharification (enzyme loading: 2.5–17.5%, and reaction volume: 550–850 µL). A total of 46 experimental conditions were evaluated and the maximum sugar yield (423 mg/g) was obtained after 18 h enzymatic hydrolysis under optimized conditions (0.25 mol/L NaOH at 202 °C for 40 min, with 12.5% of enzyme loading). Biomass compositional analyses showed that the pretreatments strongly removed lignin (up to 70%), silica (up to 80%) and promoted cellulose enrichment (25–110%). This robust design of experiments resulted in maximizing enzymatic hydrolysis efficiency of sugarcane bagasse and further indicated that this combined approach is versatile for other lignocellulosic biomasses.