Mechanism concerning the occurrence and removal of antibiotic resistance genes in composting product with ozone post-treatment. (February 2021)

• Record Type: Journal Article
• Authors: Wu, Yiqi; Chen, Zhiqiang; Wen, Qinxue; Fu, Qiqi; Bao, Huanyu
• Is Part Of: Bioresource technology. Volume 321(2021)
• Publication Date: 2021-02
• Subjects: Composting -- Ozone -- Intracellular antibiotic resistance genes -- Extracellular antibiotic resistance genes; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124433
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Ozone was adopted as a post-treatment method to remove ARGs in composting product. Ozone treatment removed the total ARGs and MGEs. Total extracellular ARGs decreased in ozone-treated sample even after 10-day storage. The reduction in intracellular 16S rRNA caused intracellular tetQ and tetW removal. The variations in extracellular ARGs after ozonation related to MGEs abundance. Abstract: The soil application of composting product will probably cause the spread of antibiotic resistance genes (ARGs) to environment, thereby it is crucial to remove ARGs in composting product. Ozone was adopted for the first time as a post-treatment method to remove the ARGs in composting product in this study. Ozone treatment significantly removed the total ARGs and mobile genetic elements (MGEs) once ozonation process finished. After 10-day storage stage, although the amount of total intracellular ARGs and MGEs increased, the total extracellular ARGs and MGEs decreased in the ozone-treated compost product. Correlation analysis revealed that the reduction in intracellular 16S rRNA contributed to intracellular tetQ and tetW removal, while the variations of other ARGs after ozonation related to MGEs abundance. Network analysis suggested that the reduction of potential host bacteria, as well as the decline in NH4 + -N and TOC after the ozonation, contributed to the intracellular ARGs removal.