The heterochronic gene Oryza sativa LIKE HETEROCHROMATIN PROTEIN 1 modulates miR156b/c/i/e levels. Issue 12 (12th August 2020)

• Record Type: Journal Article
• Authors: Cui, Yongtao; Cheng, Jingfei; Ruan, Shuang; Qi, Peipei; Liu, Wei; Bian, Hongwu; Ye, Luhuan; Zhang, Yuping; Hu, Jiang; Dong, Guojun; Guo, Longbiao; Zhang, Yijing; Qian, Qian; Hu, Xingming
• Is Part Of: Journal of integrative plant biology. Volume 62:Issue 12(2020)
• Page Start: 1839
• Page End: 1852
• Publication Date: 2020-08-12
• Subjects: Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12991
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: The juvenile‐to‐adult transition in plants involves changes in vegetative growth and plant architecture; the timing of this transition has important implications for agriculture. The microRNA miR156 regulates this transition and shoot maturation in plants. In Arabidopsis thaliana, deposition of histone H3 trimethylation on lysine 27 (H3K27me3, a repressive mark) at the MIR156A/C loci is regulated by Polycomb Repressive Complex 1 (PRC1) or PRC2, depending on the developmental stage. The levels of miR156 progressively decline during shoot maturation. The amount of H3K27me3 at MIR156A/C loci affects miR156 levels; however, whether this epigenetic regulation is conserved remains unclear. Here, we found that in rice ( Oryza sativa ), the putative PRC1 subunit LIKE HETEROCHROMATIN PROTEIN 1 (OsLHP1), with the miR156–SQUAMOSA PROMOTER BINDING PROTEIN‐LIKE (SPL) module, affects developmental phase transitions. Loss of OsLHP1 function results in ectopic expression of MIR156B/C/I/E, phenocopy of miR156 overexpression, and reduced H3k27me3 levels at MIR156B/C/I/E . This indicates that OsLHP1 has functionally diverged from Arabidopsis LHP1 . Genetic and transcriptome analyses of wild‐type, miR 156b/c‐ overexpression, and Oslhp1‐2 mutant plants suggest that OsLHP1 acts upstream of miR156 and SPL during the juvenile‐to‐adult transition. Therefore, modifying the OsLHP1–miR156–SPL pathway may enable alteration of the vegetative period and plant architecture. Abstract : In this study, we use mutant phenotype observation, analyzing genetic relationship with miR156s by epistasis genetic test, genome‐wide H3K27me3 ChIP‐sequencing, and transcriptome profiling to show that OsLHP1 has diverged in function from its ortholog in Arabidopsis . OsLHP1 mediate miR156b/c/i/e expression to ensure normal vegetative phase transitions in rice.