Investigation of Viral and Host Chromatin by ChIP‐PCR or ChIP‐Seq Analysis. Issue 1 (13th February 2018)

• Record Type: Journal Article
• Authors: Günther, Thomas; Theiss, Juliane M.; Fischer, Nicole; Grundhoff, Adam
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 40:Issue 1(2016)
• Page Start: 1E.10.1
• Page End: 1E.10.21
• Publication Date: 2018-02-13
• Subjects: chromatin -- ChIP -- ChIP‐PCR -- ChIP‐seq -- viral chromatin -- next‐generation sequencing; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/9780471729259.mc01e10s40
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Complex regulation of viral transcription patterns and DNA replication levels is a feature of many DNA viruses. This is especially true for those viruses which establish latent or persistent infections (e.g., herpesviruses, papillomaviruses, polyomaviruses, or adenovirus), as long‐term persistence often requires adaptation of gene expression programs and/or replication levels to the cellular milieu. A key factor in the control of such processes is the establishment of a specific chromatin state on promoters or replication origins, which in turn will determine whether or not the underlying DNA is accessible for other factors that mediate downstream processes. Chromatin immunoprecipitation (ChIP) is a powerful technique to investigate viral chromatin, in particular to study binding patterns of modified histones, transcription factors or other DNA‐/chromatin‐binding proteins that regulate the viral lifecycle. Here, we provide protocols that are suitable for performing ChIP‐PCR and ChIP‐Seq studies on chromatin of large and small viral genomes.© 2016 by John Wiley & Sons, Inc.