HDL Nanoparticles Have Wound Healing and Anti‐Inflammatory Properties and Can Topically Deliver miRNAs. Issue 12 (30th September 2020)

• Record Type: Journal Article
• Authors: Wang, Junyi; Calvert, Andrea E.; Kaplan, Nihal; McMahon, Kaylin M.; Yang, Wending; Lu, Kurt Q.; Peng, Han; Thaxton, Colby Shad; Lavker, Robert M.
• Is Part Of: Advanced therapeutics. Volume 3:Issue 12(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-09-30
• Subjects: alkali burns -- corneal epithelium -- diabetic corneas -- EphA2 -- microRNA; Therapeutics -- Periodicals; Pharmaceutical technology -- Periodicals; Pharmacogenetics -- Periodicals; 615.5
• DOI: 10.1002/adtp.202000138
• Languages: English
• ISSNs: 2366-3987

Abstract

Abstract: microRNAs regulate numerous biological processes, making them potential therapeutic agents. Problems with delivery and stability of these molecules have limited their usefulness as treatments. It is demonstrated that synthetic high‐density lipoprotein nanoparticles (HDL NPs) topically applied to the intact ocular surface are taken up by epithelial and stromal cells. microRNAs complexed to HDL NPs (miR‐HDL NPs) are similarly taken up by cells and tissues and retain biological activity. Topical treatment of diabetic mice with either HDL NPs or miR‐HDL NPs significantly improves corneal reepithelialization following wounding compared with controls. Mouse corneas with alkali burn‐induced inflammation, topically treated with HDL NPs, display clinical, morphological, and immunological improvement. These results yield a novel HDL NP‐based eye drop for patients with compromised wound healing ability (diabetics) and/or corneal inflammatory diseases (e.g., dry eye). Abstract : An eye drop composed of synthetic, functional high‐density lipoprotein nanoparticles (HDL NPs) in buffered saline enhances reepithelialization following corneal wounding and acts as an anti‐inflammatory agent following a chemical burn. Also, HDL NPs can be complexed with miRNAs enabling their delivery to corneal cells where the miRNAs maintain biological activity. HDL NPs have vast therapeutic potential in an ocular setting.