YEATS2 is a target of HIF1α and promotes pancreatic cancer cell proliferation and migration. Issue 3 (4th August 2020)

• Record Type: Journal Article
• Authors: Zeng, Zhirui; Lei, Shan; He, Zhiwei; Chen, Tengxiang; Jiang, Jianxin
• Is Part Of: Journal of cellular physiology. Volume 236:Issue 3(2021)
• Page Start: 2087
• Page End: 2098
• Publication Date: 2020-08-04
• Subjects: cell migration -- cell proliferation -- HIF1α -- hypoxia -- pancreatic cancer -- YEATS; Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.29995
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract: Hypoxia is involved in the development of pancreatic cancer (PC). The responses of hypoxia‐associated genes and their regulated mechanisms are largely unknown. In this study, through bioinformatic analysis and quantitative real‐time polymerase chain reaction, the YEATS domain containing 2 ( YEATS2 ) was determined to be a key hypoxia‐associated gene. It was increased in PC cells under hypoxia, upregulated in PC tissues, and predicted poor outcome. YEATS2 inhibition decreased the proliferation and migration of PC cells under both normoxia and hypoxia in vitro as well as proliferation and metastasis in vivo. We found that hypoxia‐inducible factor 1α (HIF1α) regulated the expression of YEATS2 via binding to the hypoxia response element (HRE) of YEATS2 and coexpressed with YEATS2 in PC tissues. Overexpression of YEATS2 blocked the inhibitory effects of HIF1α silence on PC cell proliferation and migration under hypoxia. Collectively, our study revealed that YEATS2 is a target gene of HIF1α and promotes PC development under hypoxia. Abstract : Hypoxia‐inducible factor 1α (HIF1α) regulated the expression of YEATS domain containing 2 (YEATS2) via binding to the hypoxia response element (HRE) of YEATS2 and coexpressed with YEATS2 in pancreatic cancer (PC) tissues. Overexpression of YEATS2 blocked the inhibitory effects of HIF1α silence on PC cell proliferation and migration under hypoxia. Collectively, our study revealed that YEATS2 is a target gene of HIF1α and promotes PC development under hypoxia.