A novel dipeptide derived from porcine liver hydrolysate induces recovery from physical fatigue in a mouse model. (January 2021)

• Record Type: Journal Article
• Authors: Nakagawasai, Osamu; Yamada, Kotaro; Sakuma, Wakana; Takahashi, Kohei; Odaira, Takayo; Yamagata, Ryota; Nemoto, Wataru; Ejima, Akika; Sato, Kenji; Tan-No, Koichi
• Is Part Of: Journal of functional foods. Volume 76(2021)
• Publication Date: 2021-01
• Subjects: Porcine liver hydrolysate -- Dipeptide -- Physical fatigue -- AMP-activated protein kinase; ANOVA analysis of variance -- AMP adenosine monophosphate -- AMPK adenosine monophosphate-activated protein kinase -- i.p. intraperitoneal -- LH liver hydrolysate -- p- phospho -- SEM standard error of the mean; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104312
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Hydrophobic peptides with an amino group in LH exerted anti-fatigue effects. Asp-Leu (Lβ), Asp-Phe (Dα), and Asp-Phe (Lα) exerted anti-fatigue effects. These aspartic peptide isomers induced recovery from fatigue at 0.03 mg/kg. Asp-Phe (Lα) enhanced p-AMPK levels in the liver after forced walking. Abstract: The present study was conducted to identify anti-fatigue peptides in porcine liver hydrolysate (LH) and to evaluate their effects. Peptides in LH were fractionated into hydrophilic and hydrophobic fractions and further into peptides with amino groups (Pep-NH2 ) and pyroglutamyl peptide fractions (pE). Low dose of hydrophobic Pep-NH2 fraction reversed the decrease in locomotor activity after forced walking in a mouse model upon intraperitoneal (i.p.) administration. The anti-fatigue effects of i.p. injection of Asp-Val, Asp-Leu, and Asp-Phe with α and β peptide bonds and D- and L- asparatyl residue, which appeared in blood after oral administration of LH, were examined. In particular, Asp-Leu (Lβ), Asp-Phe (Dα), and Asp-Phe (Lα) exerted anti-fatigue effects at a low concentration (0.03 mg/kg). We found that Asp-Phe (Lα), but not other dipeptides, activated adenosine monophosphate-activated protein kinase (AMPK) in the liver, indicating that Asp-Phe (Lα) could induce recovery from fatigue via AMPK activation.