A iridium(III) complex-based 'turn-on' fluorescent probe with two recognition site for rapid detection of thiophenol and its application in water samples and human serum. (1st January 2021)

• Record Type: Journal Article
• Authors: Xiao, Lin; Zhang, Daobin; Zhang, Jiulong; Pu, Shouzhi
• Is Part Of: Tetrahedron. Volume 77(2021)
• Publication Date: 2021-01-01
• Subjects: Iridium complex -- Thiophenol -- Near-infrared -- Real water samples; Chemistry, Organic -- Periodicals; 547.005
• DOI: 10.1016/j.tet.2020.131738
• Languages: English
• ISSNs: 0040-4020

Abstract

Abstract: A new iridium(Ⅲ) complex-based near-infrared 'turn-on' fluorescent probe Ir-DNBS was synthesized for fast (9 min), highly selective and sensitive (LOD: 62.5 nM) thiophenol detection. Photophysical and spectral characterization (NMR, HRMS) results demonstrated that the recognization mechanism of probe for thiophenol was based on thiolate-mediated nucleophilic elimination reaction (SN Ar). Upon addition with thiophenol to PBS/DCM/DMSO solution (pH = 7.4) of probe Ir-DNBS, obvious orange red fluorescence with maximum emission wavelength at 620 nm was observed with a large Stokes shift (200 nm) and long lifetime (135 ns). The practical utility of probe Ir-DNBS was also investigated by thiophenol detection in real water samples and human serum. Graphical abstract: Image 1 Highlights: A "turn on" probe based on Ir(III) complex with two recognition units (DNBS) for detection of thiphenol was synthesized. It could be used to detect thiophenol in real water samples and human serum. It could apply in wide pH range (7–11) of environments and physiological conditions.