Procoagulant properties of bare and highly PEGylated vinyl-modified silica nanoparticles. (August 2010)

• Record Type: Journal Article
• Authors: Tavano, Regina; Segat, Daniela; Reddi, Elena; Kos, Janko; Rojnik, Matija; Kocbek, Petra; Iratni, Selma; Scheglmann, Dietrich; Colucci, Mario; Echevarria, Iria Maria Rio; Selvestrel, Francesco; Mancin, Fabrizio; Papini, Emanuele
• Is Part Of: Nanomedicine. Volume 5:Number 6(2010)
• Page Start: 881
• Page End: 896
• Publication Date: 2010-08
• Subjects: blood coagulation -- Hageman factor -- HUVEC -- liposomes -- monocytes -- ORMOSIL nanoparticles -- PEGylation -- platelets -- PLGA nanoparticles -- synthetic amorphous silica nanoparticles -- tissue factor; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm.10.65
• Languages: English
• ISSNs: 1743-5889

Abstract

Aims: Undesired alterations of the blood clotting balance may follow the intravascular injection of nanotherapeutics/diagnostics. Here, we tested the procoagulant activity of synthetic amorphous silica (SAS) and organically modified silica (ORMOSIL) nanoparticles (NPs) and whether a high-density polyethylene glycol coating minimizes these effects.Materials & methods: Hageman factor- and tissue factor-dependent activation of human blood/plasma coagulation, and binding to human monocytes, endothelial cells and platelets were quantified in vitro using naked and PEGylated ORMOSIL-NPs. Their effects were compared with those of SAS-NPs, present in many industrial products, and of poly(lactic-co-glycolic acid)- and small unilamellar vesicles-NPs, already approved for use in humans.Results: Both SAS-NPs and ORMOSIL-NPS presented a significant procoagulant activity. However, highly PEGylated ORMOSIL-NPs were particularly averse to the interaction with the soluble factors and cellular elements that may lead to intravascular blood coagulation. Conclusion: Stealth, highly PEGylated ORMOSIL-NPs with a poor procoagulant activity can be used as starting blocks to design hemocompatible nanomedical-devices.