A Fast and Reliable Method to Isolate Human Placental Macrophages. (24th May 2019)

• Record Type: Journal Article
• Authors: Mezouar, Soraya; Ben Amara, Amira; Chartier, Céline; Gorvel, Laurent; Mege, Jean‐Louis
• Editors: Coligan, John E.; Bierer, Barbara E.; Margulies, David H.; Shevach, Ethan M.; Strober, Warren
• Is Part Of: Current protocols in immunology. Volume 125(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-05-24
• Subjects: CD14 selection -- enzymatic digestion -- Ficoll cushion -- flow cytometry -- placental macrophages; Immunology -- Laboratory manuals; Medical protocols; Immunologic Techniques; Medical protocols; Immunology; Laboratory manuals; Reference works; 571.96028
• DOI: 10.1002/cpim.77
• Languages: English
• ISSNs: 1934-3671

Abstract

Abstract: Macrophages are specialized cells involved in recognition, uptake, and destruction of microorganisms. Human placental macrophages are poorly investigated because of the lack of a convenient protocol for their isolation. Here, we present a straightforward and reliable method to isolate macrophages from full‐term human placentas. After enzymatic digestion of placental tissue and centrifugation of the cell suspension on a Ficoll cushion, placental macrophages are selected using magnetic beads coated with anti‐CD14 antibodies. Isolated cells are characterized by flow cytometry. Ninety eight percent of isolated CD14 + placental macrophages also express the macrophage marker CD68. Thus, this efficient and reliable method yields placental macrophages at high purity and sufficient quantity for functional studies. © 2019 by John Wiley & Sons, Inc.