Isobaric protein and peptide quantification: perspectives and issues. (October 2010)

• Record Type: Journal Article
• Authors: Treumann, Achim; Thiede, Bernd
• Is Part Of: Expert review of proteomics. Volume 7:Number 5(2010)
• Page Start: 647
• Page End: 653
• Publication Date: 2010-10
• Subjects: IPTL -- isobaric peptide termini labeling -- isobaric tag for relative and absolute quantification -- isobaric tagging -- iTRAQ -- multiplexing -- quantitative proteomics -- tandem mass tagging -- TMT; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/epr.10.29
• Languages: English
• ISSNs: 1478-9450

Abstract

An important challenge for proteomics is the ability to compare protein levels across biological samples. Since their introduction, isotopic and isobaric peptide labeling have played an important role in relative quantitative comparisons of proteomes. One important drawback of most of the isotopic-labeling techniques is an increase in sample complexity. This problem was successfully addressed with the construction of isobaric labeling strategies, such as isobaric tag for relative and absolute quantification (iTRAQ), tandem mass tagging, the cleavable isobaric affinity tag, dimethylated leucines and isobaric peptide termini labeling. Furthermore, numerous applications for multiplexing using iTRAQ and tandem mass tagging have been reported.