Natural glycan microarrays. (October 2010)

• Record Type: Journal Article
• Authors: Lonardi, Emanuela; Balog, Crina IA; Deelder, André M; Wuhrer, Manfred
• Is Part Of: Expert review of proteomics. Volume 7:Number 5(2010)
• Page Start: 761
• Page End: 774
• Publication Date: 2010-10
• Subjects: derivatization -- glycan microarray -- high-throughput screening -- immobilization -- mass spectrometry -- natural sources -- SPR -- surface plasmon resonance; Proteins -- Biotechnology -- Periodicals; Proteomics -- Periodicals; 572.6
• DOI: 10.1586/epr.10.41
• Languages: English
• ISSNs: 1478-9450

Abstract

Glycan microarrays are emerging as increasingly used screening tools with a high potential for unraveling protein–carbohydrate interactions: probing hundreds or even thousands of glycans in parallel, they provide the researcher with a vast amount of data in a short time-frame, while using relatively small amounts of analytes. Natural glycan microarrays focus on the glycans' repertoire of natural sources, including both well-defined structures as well as still-unknown ones. This article compares different natural glycan microarray strategies. Glycan probes may comprise oligosaccharides from glycoproteins as well as glycolipids and polysaccharides. Oligosaccharides may be purified from scarce biological samples that are of particular relevance for the carbohydrate-binding protein to be studied. We give an overview of strategies for glycan isolation, derivatization, fractionation, immobilization and structural characterization. Detection methods such as fluorescence analysis and surface plasmon resonance are summarized. The importance of glycan density and multivalency is discussed. Furthermore, some applications of natural glycan microarrays for studying lectin and antibody binding are presented.