Generation of Recombinant Vaccinia Viruses. (13th February 2018)

• Record Type: Journal Article
• Authors: Wyatt, Linda S.; Earl, Patricia L.; Moss, Bernard
• Editors: Coligan, John E.; Dunn, Ben M.; Speicher, David W.; Wingfield, Paul T.
• Is Part Of: Current protocols in protein science. Volume 89(2017)
• Page Start: 5.13.1
• Page End: 5.13.18
• Publication Date: 2018-02-13
• Subjects: Proteins -- Laboratory manuals; Proteins; Clinical Laboratory Techniques; Genetic Techniques; Immunologic Techniques; Proteins; Laboratory manuals; 572.6028
• DOI: 10.1002/cpps.33
• Languages: English
• ISSNs: 1934-3655

Abstract

Abstract: This unit describes how to infect cells with vaccinia virus and then transfect them with a plasmid‐transfer vector or PCR fragment to generate a recombinant virus. Selection and screening methods used to isolate recombinant viruses and a method for the amplification of recombinant viruses are described. Finally, a method for live immunostaining that has been used primarily for detection of recombinant modified vaccinia virus Ankara (MVA) is presented. © 2017 by John Wiley & Sons, Inc.