Maceration-Mediated Liquid–Liquid Extraction and Reverse-Phase High-Performance Liquid Chromatography-Based Pragmatic Analysis of Silybins. (24th July 2020)

• Record Type: Journal Article
• Authors: Hasany, Syeda Mariam; Huma, Rahila; Akram, Sumia; Ashraf, Rizwan; Mushtaq, Muhammad
• Is Part Of: Journal of chromatographic science. Volume 58:Number 8(2020)
• Page Start: 779
• Page End: 787
• Publication Date: 2020-07-24
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmaa035
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: This study presents a pragmatic and easily scalable maceration-mediated liquid–liquid extraction (MMLLE) and reverse-phase high-performance liquid chromatography (RP-HPLC)-based determination of Silybins from plant material ( Curcuma longa L.). The processing of calibration standards revealed that the RP-HPLC method was linear over a concentration range of 1–100 μg/mL with regression coefficient ( R 2 ) > 0.9950, limit of detection 0.02 μg/mL and limit of quantification <0.07 μg/mL. The optimum chromatographic conditions resolved Silybin A, Silybin B, Isosilybin A and Isosilybin B within 5 min of analysis time. The reproducible recovery rates of spiked flavonolignans (96.24–115.40%) from quality controls established the effectiveness of MMLLE procedure prior to HPLC determination. The real-time analysis revealed the presence of silybins in C. longa roots. The results further endorse that MMLLE prior to chromatographic determination may provide a more pragmatic analytical solution for the analysis/isolation of silybins.