24, 25-Dihydroxyvitamin D3 and Vitamin D Status of Community-Dwelling Black and White Americans. (6th January 2020)

• Record Type: Journal Article
• Authors: Berg, Anders H; Powe, Camille E; Evans, Michele K; Wenger, Julia; Ortiz, Guillermo; Zonderman, Alan B; Suntharalingam, Pirianthini; Lucchesi, Kathryn; Powe, Neil R; Karumanchi, S Ananth; Thadhani, Ravi I
• Is Part Of: Clinical chemistry. Volume 61:Number 6(2015)
• Page Start: 877
• Page End: 884
• Publication Date: 2020-01-06
• Subjects: Clinical chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Biochimie -- Périodiques; Diagnostics biologiques -- Périodiques; Biochemistry; Clinical chemistry; Pharmaceutical chemistry; Biochemistry; Laboratory Techniques and Procedures; Klinische chemie; Periodicals; 616.075605
• DOI: 10.1373/clinchem.2015.240051
• Languages: English
• ISSNs: 0009-9147

Abstract

Abstract: BACKGROUND: 24, 25-Dihydroxyvitamin D [24, 25(OH)2 D] is a metabolite of 25-hydroxyvitamin D (25D). Blacks frequently have low total 25D without manifestations of vitamin D deficiency, suggesting that total serum 25D may incorrectly reflect vitamin D status in different racial groups. The ratio of serum 24, 25(OH)2 D to 25D [vitamin D metabolite ratio (VMR)] represents a new candidate biomarker for vitamin D status. METHODS: We measured 24, 25(OH)2 D3 and 25D3 by mass spectrometry in a random community cohort of black (n = 212) and white (n = 164) Americans to evaluate VMR as a marker for vitamin D status. We measured parathyroid hormone concentrations by immunoassay to compare VMR and 25D3 against a physiological indicator of vitamin D deficiency. RESULTS: Serum 24, 25(OH)2 D3 strongly correlated with 25D3 in both black and white study participants ( r = 0.90, P < 0.001 and r = 0.86, P < 0.001 respectively). Blacks had lower mean 25D3 than whites [17.0 (7.8) vs 27.5 (11.3) ng/mL; 42.4 (19.5) vs 68.6 (28.2) nmol/L, P < 0.001] and lower mean 24, 25(OH)2 D3 [2.1 (1.3) vs 3.6 (2.0) ng/mL; 5.1 (3.1) vs 8.7 (4.8) nmol/L, P < 0.001]. In contrast to total 25D3 concentrations, mean VMR values were similar in blacks and whites [11.9 (4.0) vs 12.5 (3.4), P = 0.16, respectively] and were negatively correlated with parathyroid hormone concentrations in both races ( r s = −0.26, P < 0.001, and r s = −0.25, P < 0.001, respectively). CONCLUSIONS: Our results provide further evidence that measurement of total 25D for assessment of vitamin D status in patients of African descent deserves reevaluation and suggest that alternative measures such as VMR should be considered.