Type 1 Diabetic Serum Interferes with Pancreatic β-cell Ca2+ -Handling. Issue 6 (20th November 2007)

• Record Type: Journal Article
• Authors: Dekki, N.; Nilsson, R.; Norgren, S.; Rössner, S. M.; Appelskog, I.; Marcus, C.; Simell, O.; Pugliese, A.; Alejandro, R.; Ricordi, C.; Berggren, P. O.; Juntti-Berggren, L.
• Is Part Of: Bioscience reports. Volume 27:Issue 6(2007)
• Page Start: 321
• Page End: 326
• Publication Date: 2007-11-20
• Subjects: Pancreatic β-cell -- Intracellular Ca2+ -- Type 1 diabetes -- Serum -- Autoantibodies; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1007/s10540-007-9055-y
• Languages: English
• ISSNs: 0144-8463

Abstract

Abstract : The aim of this study was to clarify the frequency of patients with type 1 diabetes that have serum that increases pancreatic β-cell cytoplasmic free Ca 2+ concentration, [Ca 2+ ]i, and if such an effect is also present in serum from first-degree relatives. We also studied a possible link between the serum effect and ethnic background as well as presence of autoantibodies. Sera obtained from three different countries were investigated as follows: 82 Swedish Caucasians with newly diagnosed type 1 diabetes, 56 Americans with different duration of type 1 diabetes, 117 American first-degree relatives of type 1 diabetic patients with a mixed ethnic background and 31 Caucasian Finnish children with newly diagnosed type 1 diabetes. Changes in [Ca 2+ ]i, upon depolarization, were measured in β-cells incubated overnight with sera from type 1 diabetic patients, first-degree relatives or healthy controls. Our data show that there is a group constituting approximately 30% of type 1 diabetic patients of different gender, age, ethnic background and duration of the disease, as well as first-degree relatives of type 1 diabetic patients, that have sera that interfere with pancreatic β-cell Ca 2+ -handling. This effect on β-cell [Ca 2+ ]i could not be correlated to the presence of autoantibodies. In a defined subgroup of patients with type 1 diabetes and first-degree relatives a defect Ca 2+ -handling may aggravate development of β-cell destruction.