Evaluation of plant stress due to plasma-generated reactive oxygen and nitrogen species using electrolyte leakage. (29th December 2020)

• Record Type: Journal Article
• Authors: Takashima, Keisuke; Ahmad Nor, Ahmad Shahir bin; Ando, Sugihiro; Takahashi, Hideki; Kaneko, Toshiro
• Is Part Of: Japanese journal of applied physics. Volume 60:Number 1(2021)
• Publication Date: 2020-12-29
• Subjects: RONS stress measure -- Atmospheric pressure air plasma -- K + ion leakage -- Electrolyte leakage -- Plasma agriculture; Physics -- Periodicals; 621.05
• DOI: 10.35848/1347-4065/abcf5b
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: The aim of this paper is to highlight the importance of plant-stress measurement, allowing the quantitative evaluation of plant stress due to plasma-generated gas-phase reactive oxygen and nitrogen species (RONS). It is found that electrolyte leakage from Arabidopsis thaliana leaves, detectable within one hour, tends to reflect gas-phase RONS exposure but does not correlate well with subsequently observed wilting. Ion chromatograph analysis of the leaked electrolyte indicates that selective leakage of potassium ions (K + ) accounts for nearly 80% of the measured leaked ions. This selective and rapid K + leakage due to the RONS exposure stress can be interpreted as part of the plant's response, and is potentially involved in subsequent plasma-induced phenomena but can hardly be explained by physical damage. Thus, electrolyte leakage as a plant stress response can be a useful RONS stress measure, particularly for plasma-based agricultural applications.