Screening for the human neutrophil antigen‐2 deficiency by a multiplex one‐tube polymerase chain reaction assay. (4th March 2020)

• Record Type: Journal Article
• Authors: Gong, Jiwu; Rao, Meiying; Jia, Zhonghua; Ma, Na; Sun, Lifang; Yu, Xiaonan; Chen, Feng; Xie, Jue; Gong, Hongwei; Fu, Qiang; Cao, Chen; Chen, Fangfang; Zhao, Tongmao
• Is Part Of: ISBT science series. Volume 15:Number 2(2020)
• Page Start: 286
• Page End: 291
• Publication Date: 2020-03-04
• Subjects: Chinese population -- HNA‐2 deficiency -- HNA‐2 genotyping -- human neutrophil antigen; Blood -- Periodicals; Blood -- Transfusion -- Periodicals; Immunohematology -- Periodicals; Immunopathology -- Periodicals; 615.39
• DOI: 10.1111/voxs.12546
• Languages: English
• ISSNs: 1751-2816

Abstract

Abstract : Objectives: The aim of this study was to develop a rapid method for human neutrophil antigen‐2 (HNA‐2) genotyping and use it for fast screening for the HNA‐2 deficiency phenotype in the Chinese population. Background: HNA‐2 is encoded by the CD177 gene, and a single nucleotide polymorphism (SNP) at cDNA position 787 ( CD177 *787 A > T) creates a stop codon, which is responsible for the HNA‐2 deficiency phenotype. Recently, the SNP c.787A > T has been demonstrated to be the primary genetic mechanism for HNA‐2 deficiency. Methods: This study focused on determination of CD177 SNP c.787A > T. We designed a set of allele‐ and sequence‐specific primers (SSP), which were used to develop a one‐tube polymerase chain reaction (PCR) assay for HNA‐2 genotyping. The HNA‐2 genotype of a total of 2185 Chinese blood donors was determined. Results: The validation of this HNA‐2 genotyping assay was confirmed by sequencing analysis. The HNA‐2 two alleles, CD177* 787A and 787T, can be simultaneously determined in a single tube PCR. Thirteen individuals with the CD177* 787AT genotype were detected in this study. The CD177* 787TT homozygous genotype was absent, suggesting that HNA‐2 deficiency is rare in the Chinese population. Conclusions: The validated assay described in this study is a rapid and reliable method for HNA‐2 genotyping, which may be used for fast screening for HNA‐2 deficiency.