Quality assurance and authentication of herbal drug (Argyrolobium roseum and Viola stocksii) through comparative light and scanning electron microscopy. Issue 1 (17th August 2020)

• Record Type: Journal Article
• Authors: Ahmed, Sidra Nisar; Ahmad, Mushtaq; Yaseen, Ghulam; Zafar, Muhammad; Rashid, Sofia; Nazir, Abdul; Sultana, Shazia; Anjum, Farida; Ozdemir, Fethi Ahmet; Kilic, Omer
• Is Part Of: Microscopy research and technique. Volume 84:Issue 1(2021)
• Page Start: 28
• Page End: 37
• Publication Date: 2020-08-17
• Subjects: crude herbal drugs -- leaf anatomy -- pollen -- quality assurance -- scanning electron microscopy; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23560
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: The quality assurance and authentication of crude herbal drugs play important role in the effective therapeutic effect of herbal drug and their products. There are many reported problems in quality assurance of herbal crude drugs concerning to their correct identification. The present study was designed with the aim to document the authentication and quality assurance of the herbal crude drugs ( Argyrolobium roseum and Viola stocksii ) thorough light microscopy (LM) and scanning electron microscopy (SEM). The detailed foliar anatomical studies showed polygonal epidermal cells having anticlinal walls in Argyrolobium roseum while rounded epidermal cells were observed in Viola stocksii . The anomocytic stomata type was observed in Argrolobium roseum while actinocytic was noticed in Viola stocksii . The pollen of studied species appeared as tricolporate showing reticulate exine sculpturing in Argrolobium roseum while fine perforations were recorded in Viola stocksii . Furthermore, quantitative analysis of phenols, flavonoids, and antioxidant activity showed high flavonoid and phenol content in Argyrolobium roseum as compared with Viola stocksii . It was observed that Argyrolobium roseum was discriminated from the Viola stocksii based on the leaf and pollen micromorphological traits by using LM and SEM techniques. It was concluded that LM and SEM techniques were found useful for the quality assurance of botanicals and their authentication. Abstract : Polygonal epidermal cells having anticlinal walls were found in Argyrolobium roseum and rounded were observed in Viola stocksii . The pollen were tricolporate showing reticulate sculpturing in Argrolobium roseum while perforations in Viola stocksii LM and SEM are found to be very useful tools for quality assurance of herbal drugs ( Argyrolobium roseum and Viola stocksii )