A Flexible DNA Biosensor for Breast Cancer Marker BRCA1 Detection. Issue 48 (28th December 2020)

• Record Type: Journal Article
• Authors: Wang, Qi; Zhang, Huanyu; Shen, Xiaohui; Wang, Fenghe; Mao, Chun
• Is Part Of: ChemistrySelect. Volume 5:Issue 48(2020)
• Page Start: 15259
• Page End: 15263
• Publication Date: 2020-12-28
• Subjects: flexible electrode -- breast cancer gene detection -- DNA biosensor -- conductive polymeric film; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/slct.202003933
• Languages: English
• ISSNs: 2365-6549

Abstract

Abstract: Early detection of breast cancer is of great significance to improve the prognosis and survival rate of breast cancer. However, how to detect breast cancer 1 gene mutation (BRCA1) efficiently in early stage is still a great challenge. In the past few decades, more and more attention has been paid to the design of a biosensor. In this work, A method for rapid detection of specific BRCA1 using silver nanowires modified polyethylene terephthalate (PET) film as a disposable electrode was proposed. With a high aspect ratio, AgNWs distributed in the PET film were easy to form a conductive network as a flexible electrode. A well‐conductive gold sputtering layer can prevent AgNW from falling off to provide a conductive and stable electrode substrate. The Au‐HRP can effectively reflect the hybridization matching phenomenon of target. Results indicated this strategy we proposed had a low detection limit with different concentrations of complementary DNA with limit of detection of 5.3×10 −15 M in the range from 5.0×10 −10 M to 5.0×10 −7 M. Due to the high sensitivity, simplicity and cheap price, arbitrary cut, AgNWs/PET film becomes a promising electrode material for quantification of low concentration biological component in early diagnosis. Abstract : A flexible DNA biosensor was reported composed of silver nanowires decorated‐polyethylene terephthalate (AgNWs/PET) film as a disposable and flexible electrode for the rapid detection of specific BRCA1. This strategy we proposed showed a low detection limit (5.3×10 −15 M) and wide detection range (5.0×10 −10 M to 5.0×10 −7 M). Therefore, AgNWs/PET film becomes a promising electrode material for quantification of low concentration biological component in early diagnosis.