Calcium peroxide-mediated in situ formation of multifunctional hydrogels with enhanced mesenchymal stem cell behaviors and antibacterial properties. Issue 48 (16th November 2020) - Thi, Phuong Le
Abstract : CaO2 catalyzes the formation of in situ hydrogels with multifunctional properties through its decomposition into H2 O2, O2, and Ca 2+ ions. Abstract : Injectable hydrogels can serve as therapeutic vehicles and implants for the treatment of various diseases as well as for tissue repair/regeneration. In particular, the horseradish peroxidase (HRP) and hydrogen peroxide (H2 O2 )-catalyzed hydrogelation system has attracted much attention, due to its ease of handling and controllable gel properties. In this study, we introduce calcium peroxide (CaO2 ) as a H2 O2 -generating reagent to gradually supply a radical source for the HRP-catalyzed crosslinking reaction. This novel therapy can create stiff hydrogels without compromising the cytocompatibility of the hydrogels due to the use of initially high concentrations of H2 O2 . The physico-chemical properties of the hydrogels can be controlled by varying the concentrations of HRP and CaO2 . In addition, the controlled and sustained release of bioactive molecules, including H2 O2, O2, and Ca 2+ ions, from the hydrogels could stimulate the cellular behaviors (attachment, migration, and differentiation) of human mesenchymal stem cells. Moreover, the hydrogels exhibited killing efficacy against both Gram-negative and Gram-positive bacteria, dependent on the H2 O2 and Ca 2+ release amounts. These positive results suggest that hydrogels formed by HRP/CaO2 can be used as potential matrices for a wide range of biomedical applications, such as bone regeneration and infection treatment.