Genetic Manipulation of Staphylococcus aureus. Issue 1 (6th February 2014)

• Record Type: Journal Article
• Authors: Schneewind, Olaf; Missiakas, Dominique
• Editors: Coico, Richard; McBride, Alison; Quarles, John M.; Stevenson, Brian; Taylor, Ronald K.
• Is Part Of: Current protocols in microbiology. Volume 32:Issue 1(2014)
• Page Start: 9C.3.1
• Page End: 9C.3.19
• Publication Date: 2014-02-06
• Subjects: Staphylococcus aureus -- plasmid -- bursa aurealis -- transduction -- allelic replacement; Microbiology -- Laboratory manuals; Microbiology -- Technique -- Periodicals; Microbiological Techniques -- methods; Containment of Biohazards -- methods; Laboratory Infection -- prevention & control; Microbiology; Microbiology -- Technique; Fulltext; Internet Resources; Laboratory Manuals; Reference Materials; Periodicals; Laboratory manuals; 579.028
• DOI: 10.1002/9780471729259.mc09c03s32
• Languages: English
• ISSNs: 1934-8525

Abstract

Abstract: Staphylococcus aureus is a facultative anaerobic Gram‐positive coccus and a member of the normal skin flora, as well as that of the nasal passages of humans. However, S. aureus can also gain entry into the host and cause life‐threatening infections or persist as disease foci that develop into suppurative abscesses. While genetically tractable, the manipulation of S. aureus remains challenging. This unit describes methods developed in our laboratory for gene disruption by allelic replacement and transposition. We also provide a protocol for bacteriophage‐mediated transduction of mutants marked with selectable alleles and describe plasmid utilization for complementation studies. Curr. Protoc. Microbiol . 32:9C.3.1‐9C.3.19. © 2014 by John Wiley & Sons, Inc.