Simultaneous separation of artesunate and mefloquine in fixed-dose combination tablets by CZE-UV. Issue 47 (19th November 2020)

• Record Type: Journal Article
• Authors: de Souza, Jéssica Cordeiro Queiroz; Chellini, Paula Rocha; Viçosa, Alessandra Lifsitch; de Souza, Marcus Vinícius Nora; de Oliveira, Marcone Augusto Leal
• Is Part Of: Analytical methods. Volume 12:Issue 47(2020)
• Page Start: 5709
• Page End: 5717
• Publication Date: 2020-11-19
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/d0ay01755h
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : A novel method was proposed for simultaneous determination of artesunate (ATS) and mefloquine (MFQ) in fixed-dose combination tablets by capillary zone electrophoresis with simultaneous direct and indirect detection by ultraviolet (CZE-UV). Abstract : A novel method was proposed for simultaneous determination of artesunate (ATS) and mefloquine (MFQ) in fixed-dose combination tablets by capillary zone electrophoresis with simultaneous direct and indirect detection by ultraviolet (CZE-UV). The background electrolyte, consisting of 30/15 mmol L −1 TRIS/3, 5-dinitrobenzoic acid buffer at pH 8.2, a chromophore buffer, was selected taking into account a detailed study involving the effective mobility vs. pH curves of the analytes and electrolyte compounds in association with the very low molar absorptivity of ATS. Suitable separation conditions, considering voltage, temperature and buffer concentration as factors, were achieved through the 3 3 Box–Behnken design investigation. The optimum baseline separation conditions were: injection pressure of 30 mbar for 10 s, cartridge temperature of 22.5 °C and positive voltage of +30 kV. The method proved to be rapid (5 minutes), simple, selective, linear ( r 2 > 0.98), precise (relative standard deviation (RSD): ATS < 2.9% and MFQ < 2.2%) and accurate (recoveries: ATS 98.13–102.96% and MFQ 98.75–106.77%), proving to be suitable for routine quality control analysis.