Evaluation of acetone as organic modifier in SPE for bioanalytical quantitative LC––MS/MS. (February 2011)

• Record Type: Journal Article
• Authors: MacNeill, Robert; Evans, Esther
• Is Part Of: Bioanalysis. Volume 3:Number 3(2011)
• Page Start: 291
• Page End: 300
• Publication Date: 2011-02
• Subjects: Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio.10.202
• Languages: English
• ISSNs: 1757-6180

Abstract

Background: To assess the suitability of acetone as an alternative to acetonitrile in SPE under otherwise commonly used conditions, with a focus on selectivity with regard to the most abundant phospholipid class. Two representative analytes were included, leuprolide and tramadol, a peptide and a small molecule, respectively.Results: The use of acetone resulted in analogous elution profiles of all monitored compounds in the majority of conditions. The only significant difference was on silica-based C18, where acetone effected markedly enhanced elution of lysophosphatidylcholines than did acetonitrile. Unmodified silica was shown to operate in per aqueous LC mode in highly aqueous conditions.Conclusion: Acetone was established as a selectively similar and slightly more eluotropic alternative to acetonitrile in bioanalytical SPE.