Combined Genome and Transcriptome Analyses of the Ciliate Schmidingerella arcuata (Spirotrichea) Reveal Patterns of DNA Elimination, Scrambling, and Inversion. (1st September 2020)

• Record Type: Journal Article
• Authors: Smith, Susan A; Maurer-Alcalá, Xyrus X; Yan, Ying; Katz, Laura A; Santoferrara, Luciana F; McManus, George B
• Editors: Phadke, Sujal
• Is Part Of: Genome biology and evolution. Volume 12:Number 9(2020)
• Page Start: 1616
• Page End: 1622
• Publication Date: 2020-09-01
• Subjects: genome architecture -- single-cell 'omics -- tintinnid -- micronucleus -- macronucleus -- Ciliophora; Genomics -- Periodicals; Genes -- Periodicals; 572.8605
• DOI: 10.1093/gbe/evaa185
• Languages: English
• ISSNs: 1759-6653

Abstract

Abstract: Schmidingerella arcuata is an ecologically important tintinnid ciliate that has long served as a model species in plankton trophic ecology. We present a partial micronuclear genome and macronuclear transcriptome resource for S. arcuata, acquired using single-cell techniques, and we report on pilot analyses including functional annotation and genome architecture. Our analysis shows major fragmentation, elimination, and scrambling in the micronuclear genome of S. arcuata . This work introduces a new nonmodel genome resource for the study of ciliate ecology and genomic biology and provides a detailed functional counterpart to ecological research on S. arcuata.