Transcriptome aberration in marine microalgae Phaeodactylum tricornutum induced by commercial naphthenic acids. (1st January 2021)

• Record Type: Journal Article
• Authors: Zhang, Huanxin; Hu, Yanran; Yang, Likun; Lin, Zhihao; Zhao, Xinyu; Chen, Jun; Tang, Xuexi; Wang, Ying
• Is Part Of: Environmental pollution. Volume 268(2021)Part A
• Publication Date: 2021-01-01
• Subjects: Marine ecology -- Naphthenic acids -- Microalgae -- Photosynthesis -- Transcriptome alternation; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.115735
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Naphthenic acids (NAs) are cycloalkyl linear carboxylic acids primarily originating from oil sand tailings water (OSTW), and the exposure to NAs affects the photosynthesis of marine microalgaes. However, the transcriptional response upon the stress of NAs remains unclear. Herein, we investigate the transcriptome alterations of marine Phaeodactylum tricornutum induced by NAs using RNA-sequencing. In total 70 differentially expressed genes (DEGs) are enriched in seven biological process pathways, and 48 DEGs are enriched in four molecular functions. Differentially expressed genes of P. tricornutum are primarily concentrated in three photosynthesis-related pathways including the porphyrin and chlorophyll metabolism pathway, the photosynthesis pathway, and the carbon fixation pathway in photosynthetic organisms. Five DEGs such as hemE, chlM, TPI/GapC3, FbaC5, and CHLH are validated using quantitative real-time PCR assay. NAs at low concentrations affect the material energy conversion efficiency of P. tricornutum, such as chlorophyll synthesis, electron transfer efficiency, and carbon assimilation. Graphical abstract: Image 1 Highlights: Naphthenic acids (NAs) induced transcriptome alternations in Phaeodactylum tricornutum. 148 unigenes were induced by NAs at 4 and 16 mg/L NAs. Material energy conversion related genes were altered by NAs at 4 mg/L. Photosynthesis and redox related pathway were affected by NAs. Abstract : TThe photosynthesis and redox related pathway are affected by commercial naphthenic acids (NAs) on the transcriptome alterations in marine Phaeodactylum tricornutu.