Sea cucumbers in a high temperature and low dissolved oxygen world: Roles of miRNAs in the regulation of environmental stresses. (1st January 2021)

• Record Type: Journal Article
• Authors: Huo, Da; Sun, Lina; Sun, Jingchun; Zhang, Libin; Liu, Shilin; Su, Fang; Yang, Hongsheng
• Is Part Of: Environmental pollution. Volume 268(2021)Part A
• Publication Date: 2021-01-01
• Subjects: Global climate change -- MicroRNA -- Apostichopus japonicus -- Anoxia -- Heat; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.115509
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The exacerbation of global warming has driven changes in environmental factors, including water temperature and oxygen concentration. The sea cucumber Apostichopus japonicus, an economically important aquatic animal, is constantly and directly challenged by heat and hypoxia. In this study, 12 small RNA libraries were constructed for this species, and a total of 21, 26 and 22 differentially expressed (DE) miRNAs were clarified in A. japonicus under thermal (26 °C), hypoxic (2 mg/L) and the combined stresses. Comparative miRNA sequencing analysis and real-time PCR were used to identify and validate the representative miRNAs, including Aja-miR-novel-299, Aja-let-7b-3p, Aja-miR-71b-5p, Aja-miR-novel-13218 and Aja-miR-2004 in response to high temperature, and Aja-miR-92b-3p, Aja-miR-210–5p and Aja-miR-novel-26331 in response to oxygen limitation. GO and KEGG pathway analysis revealed that the potential target genes of DE-miRNAs involved in biosynthesis, metabolism, immunity, cell growth and death, translation and signaling transduction. Key DE-miRNAs with potentially targeted genes associated with heat shock and hypoxia response were also determined. These results may help explaining the role of miRNA regulation in stress resistance, as well as the potential molecular regulation mechanism of the echinoderm A. japonicus in the context of global warming. Graphical abstract: Image 1 Highlights: MiRNA profiles of sea cucumber under thermal, hypoxic and the combination were constructed. The typical DE-miRNAs in response to heat and oxygen deficiency were identified and validated. The potential target genes of DE-miRNAs mainly involved in biosynthesis, metabolism, immunity, and etc. Potential molecular regulation mechanism of the A. japonicus under global warming were provided. Abstract : High-throughput sequencing reveals the roles of miRNAs in respiratory tree from the sea cucumber Apostichopus japonicus under heat, hypoxia and a combination of the two.