Bacteria-dye combination screening: Diamine-containing BMeS-p-A dye for specific fluorescence imaging of Acinetobacterbaumannii. (February 2021)

• Record Type: Journal Article
• Authors: Kang, Sangrim; Jung, Yuna; Jung, Junyang; Park, Ki-Ho; Kim, Dokyoung
• Is Part Of: Dyes and pigments. Volume 185(2021)Part A
• Publication Date: 2021-02
• Subjects: Bacteria imaging -- Fluorescent probe -- Amine-containing dyes -- Drug-resistant bacteria -- Staining; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2020.108939
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: We propose a bacteria-dye combination screening strategy for the fluorescence staining of a specific bacteria strain. The threat of bacteria and its drug-resistance is getting ever more severe, and they are constantly harming human beings. Under these circumstances, precise identification of a specific bacteria is essential for diagnosing and finding the proper drugs for treatment. In this study, we discovered that a positively-charged diamine-containing BMeS-p-A dye has a selective staining ability of Gram-negative Acinetobacter baumannii (AB) and carbapenem-resistant AB (CRAB) over other bacteria strains. We systematically analyzed the photophysical properties, sensing abilities, and the antibiotic effect of BMeS-p-A. We utilized the BMeS-p-A for monitoring the biofilm of AB and CRAB in medical supplies; urethral tract catheters. The results of this study will facilitate further applications in a variety of medical fields. Graphical abstract: Image 1 Highlights: An approach to the bacteria-dye combination screening with positively-charged dyes was disclosed. Diamine-containing BMeS-p-A dye showed a specific fluorescence imaging ability toward A. Baumannii. A fluorescence imaging of bacteria biofilm on the urinary tract catheter was successfully demonstrated.