Development of a reverse-transcription recombinase polymerase amplification assay with a lateral flow assay for rapid detection of avian orthoavulavirus 1. (March 2021)

• Record Type: Journal Article
• Authors: Fan, Jindai; Chen, Wenxian; Zhang, Yuanyuan; Liu, Zhixiang; Li, Xiaoming; Ding, Hongxing; Yi, Lin; Chen, Jinding; Zhao, Mingqiu
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 33:Number 2(2021)
• Page Start: 308
• Page End: 312
• Publication Date: 2021-03
• Subjects: avian orthoavulavirus 1 -- lateral flow device -- recombinase polymerase amplification; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638721990122
• Languages: English
• ISSNs: 1040-6387

Abstract

Newcastle disease is an avian infectious disease caused by avian orthoavulavirus 1, also known as Newcastle disease virus (NDV). This disease has caused significant economic losses to the poultry industry worldwide. The rapid and simple detection of NDV infection is crucial to inform the appropriate control measures. We developed a reverse-transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow assay (LFA) for NDV detection. The RPA assay can be completed at 37°C within 20 min, and the RPA result can be visualized by the LFA within 5 min. The NDV RT-RPA-LFA detected NDV specifically with no cross-reactivity with other pathogens. The detection limit of NDV cDNA with our RT-RPA-LFA was 3.34 × 10 −3 ng/μL. Consequently, the RT-RPA-LFA showed good potential for the detection of NDV infection in the field, especially in resource-limited settings.