Efficient biosynthesis of exopolysaccharide from Jerusalem artichoke using a novel strain of Bacillus velezensis LT-2. Issue 6 (January 2021)

• Record Type: Journal Article
• Authors: Meng, Qiao; Lu, Chenghui; Gao, Hua; Chen, Guoxuan; Wu, Lingtian; Wu, Jinnan; Li, Sha; He, Bing-Fang
• Is Part Of: Bioscience reports. Volume 30:Issue 6(2010)
• Publication Date: 2021-01
• Subjects: Bacillus velezensis LT-2 -- Exopolysaccharide -- Jerusalem artichoke -- Non-grain fermentation -- Transcriptome sequencing; Molecular biology -- Periodicals; Cytology -- Periodicals; 572.8
• DOI: 10.1016/j.biortech.2020.124346
• Languages: English
• ISSNs: 0144-8463

Abstract

Graphical abstract: Highlights: A GRAS strain which can directly utilize inulin was isolated for EPS biosynthesis. Jerusalem artichoke was first used as a non-grain substrate for EPS production. A maximum EPS of 11.47 g/L was produced in the batch fermentation. Comparative transcriptome reveals the reasons for EPS synthesis promotion. Provided a strategy for industrial non-grain biorefining of Jerusalem artichoke. Abstract: This study focused on the non-grain biorefining of Jerusalem artichoke (JA) for exopolysaccharide (EPS) efficient production by using Bacillus velezensis LT-2. Results showed that LT-2 could directly utilize JA tuber power (JATP) for EPS production, and its EPS yield reached 11.47 ± 0.33 g/L in the simultaneous saccharification and fermentation (SSF) mode. Furthermore, the SSF mode shortened the fermentation period by 26.67% and reduced the fermentation cost by 79.41% due to the improved substrate utilization and the avoidance of inhibition effects of a high fructose concentration. Transcriptome sequencing results showed that inulin could accelerate nucleotide-sugars biosynthesis, induce EPS synthetic gene cluster transcription, and strengthen the electron transport respiratory chain and the transporter systems, thereby ensuring EPS efficient synthesis. This work exhibited a productive non-grain and environmentally friendly fermentation strategy for EPS biosynthesis, which promoted the JA industry development and created new prospects for high-value industrial products biosynthesis by using JATP.