Anaerobic reduction of high-polarity nitroaromatic compounds by electrochemically active bacteria: Roles of Mtr respiratory pathway, molecular polarity, mediator and membrane permeability. (1st January 2021)

• Record Type: Journal Article
• Authors: Xiao, Xiang; Ma, Xiao-Lin; Wang, Lu-Guang; Long, Fei; Li, Ting-Ting; Zhou, Xiang-Tong; Liu, Hong; Wu, Li-Jun; Yu, Han-Qing
• Is Part Of: Environmental pollution. Volume 268(2021)Part B
• Publication Date: 2021-01-01
• Subjects: Anaerobic bioreduction -- Molecular polarity -- Membrane permeability -- 3-Nitrobenzenesulfonate -- Shewanella oneidensis MR-1; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.115943
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Electrochemically active bacteria (EAB) are effective for the bioreduction of nitroaromatic compounds (NACs), but the exact reduction mechanisms are unclear yet. Therefore, 3-nitrobenzenesulfonate (NBS) was used to explore the biodegradation mechanism of NACs by EAB. Results show that NBS could be anaerobically degraded by Shewanella oneidensis MR-1. The generation of aminoaromatic compounds was accompanied with the NBS reduction, indicating that NBS was biodegraded via reductive approach by S. oneidensis MR-1. The impacts of NBS concentration and cell density on the NBS reduction were evaluated. The removal of NBS depends mainly on the transmembrane electron transfer of S. oneidensis MR-1. Impairment of Mtr respiratory pathway was found to mitigate the reduction of NBS, suggesting that the anaerobic biodegradation of NBS occurred extracellularly. Knocking out cymA severely impaired the extracellular reduction ability of S. oneidensis MR-1. However, the phenotype of Δ cymA mutant could be compensated by the exogenous electron mediators, implying the trans-outer membrane diffusion of mediators into the periplasmic space. This work provides a new insight into the anaerobic reduction of aromatic contaminants by EAB. Graphical abstract: Image 1 Highlights: S. oneidensis MR-1 can degrade NACs extracellularly under anaerobic conditions. Electron mediators can improve the NACs bioreduction by EAB. Transmembrane diffusion of mediators contributes to extracellular NAC reduction. Increased membrane permeability promotes electron-mediated NAC reduction. Abstract : Nitroaromatic compounds (NACs) are biodegraded by Shewanella oneidensis MR-1mainly via Mtr respiratory pathway.