Biosorption as a method of biowaste valorization to feed additives: RSM optimization. (1st January 2021)

• Record Type: Journal Article
• Authors: Kowalczyk, P.; Ligas, B.; Skrzypczak, D.; Mikula, K.; Izydorczyk, G.; Witek-Krowiak, A.; Moustakas, K.; Chojnacka, K.
• Is Part Of: Environmental pollution. Volume 268(2021)Part B
• Publication Date: 2021-01-01
• Subjects: Feed additives -- RSM -- Microelements -- Biomass -- Sorption; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2020.115937
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: The aim of this work was to prepare an innovative microelemental feed additive for laying hens, based on waste biomass from the agricultural sector (alfalfa and goldenrod after CO2 extraction in supercritical state). The process was optimized by Response Surface Methodology (RSM) and the most favourable enrichment conditions were selected for Cu(II), Mn(II) and Zn(II) ions: pH - 5, sorbate concentration of Cu(II), Mn(II), Zn(II) - 10.0 mg/L for alfalfa and 10.7 mg/L for goldenrod and biomass dose – 0.1 g/L. Physicochemical properties of biomass were studied and functional groups involved in the binding of Cu(II), Mn(II), Zn(II) ions were determined (mainly carboxylic and hydroxylic groups). An interesting and unique element of this work is the verification of the properties of prepared feed additives in conditions simulating the digestive tract of animals. The release of components in solutions simulating conditions in the intestine and stomach (pH 11 and pH 1) was tested (in vitro tests). The best desorption results were achieved at a strongly acidic pH which corresponds to the stomach environment: 9.80, 14.4% Cu(II), 69.0, 66.9% (Zn), 46.5, 31.9 Mn(II) for alfalfa and goldenrod, respectively. It was concluded that the biomass enriched with micronutrients in biosorption has the potential as a feed additive for sustainable agriculture. Graphical abstract: Image 1 Highlights: A new direction of bio-waste management is the production of feed additives. The sorption properties of waste biomass of alfalfa and goldenrod were investigated. Response Surface Methodology (RSM) was used to optimize biosorption. The bioavailability of the micronutrients was tested by in vitro simulations.