Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte. Issue 1 (January 2021)

• Record Type: Journal Article
• Authors: Amobonye, Ayodeji; Bhagwat, Prashant; Singh, Suren; Pillai, Santhosh
• Is Part Of: Fungal biology. Volume 125:Issue 1(2021)
• Page Start: 39
• Page End: 48
• Publication Date: 2021-01
• Subjects: Beauveria bassiana -- Endoglucanase -- Xylanase -- Optimisation -- Saccharification -- Sugarcane bagasse; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2020.10.003
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: This study was undertaken to explore alternative applications of the widely known entomopathogenic/endophytic fungus, Beauveria bassiana, besides its sole use as a biocontrol agent. B. bassiana SAN01, was investigated for the production of two glycoside hydrolases, xylanase and endoglucanase under submerged conditions. Among the different biomass tested, wheat bran provided the best results for both xylanase and endoglucanase, and their production levels were further enhanced using response surface methodology. Under optimised conditions, heightened yields of 1061 U/ml and 23.03 U/ml were observed for xylanase and endoglucanase, respectively, which were 3.44 and 1.35 folds higher than their initial yields. These are the highest ever production levels reported for xylanase and endoglucanase from any B. bassiana strain or any known entomopathogenic fungi. Furthermore, the efficacy of xylanase/endoglucanase cocktail in the saccharification of sugarcane bagasse was evaluated. The highest amount of reducing sugar released from the pretreated biomass by the action of the crude Beauveria enzyme cocktail was recorded at 30°C after 8 h incubation. The significant activities of the hydrolytic enzymes recorded with B. bassiana in this study thus present promising avenues for the use of the entomopathogen as a new source of industrial enzymes and by extension, other biotechnological applications. Graphical abstract: Image 1 Highlights: Utilization of various agricultural biomass for enzyme production by B. bassiana . Highest xylanase and endoglucanase production level for an entomopathogenic fungus. Alternative industrial application of B. bassiana in biomass valorisation to reducing sugars.