Epithelial cell detachment by Porphyromonas gingivalis biofilm and planktonic cultures. Issue 4 (20th April 2016)

• Record Type: Journal Article
• Authors: Huang, Lijia; van Loveren, Cor; Ling, Junqi; Wei, Xi; Crielaard, Wim; Deng, Dong Mei
• Is Part Of: Biofouling. Volume 32:Issue 4(2016)
• Page Start: 489
• Page End: 496
• Publication Date: 2016-04-20
• Subjects: Cytotoxicity -- detachment -- gingival epithelial cells -- gingipain -- glycosyltransferase -- quantitative PCR; Marine fouling organisms -- Periodicals; Materials -- Biodeterioration -- Periodicals; Fouling -- Periodicals; Biodegradation -- Periodicals; Environmental Microbiology -- Periodicals; Water Microbiology -- Periodicals; 576.165
• DOI: 10.1080/08927014.2016.1148693
• Languages: English
• ISSNs: 0892-7014

Abstract

Abstract: Porphyromonas gingivalis is present as a biofilm at the sites of periodontal infections. The detachment of gingival epithelial cells induced by P. gingivalis biofilms was examined using planktonic cultures as a comparison. Exponentially grown planktonic cultures or 40-h biofilms were co-incubated with epithelial cells in a 24-well plate for 4 h. Epithelial cell detachment was assessed using imaging. The activity of arginine-gingipain (Rgp) and gene expression profiles of P. gingivalis cultures were examined using a gingipain assay and quantitative PCR, respectively. P. gingivalis biofilms induced significantly higher cell detachment and displayed higher Rgp activity compared to the planktonic cultures. The genes involved in gingipain post-translational modification, but not rgp genes, were significantly up-regulated in P. gingivalis biofilms. The results underline the importance of including biofilms in the study of bacterial and host cell interactions.