A fast and green reversed‐phase HPLC method with fluorescence detection for simultaneous determination of amlodipine and celecoxib in their newly approved fixed‐dose combination tablets. Issue 16 (22nd June 2020)

• Record Type: Journal Article
• Authors: Abdel Hamid, Mohamed A.; Mabrouk, Mokhtar M.; Michael, Mary A.
• Is Part Of: Journal of separation science. Volume 43:Issue 16(2020)
• Page Start: 3197
• Page End: 3205
• Publication Date: 2020-06-22
• Subjects: amlodipine -- celecoxib -- eco‐friendly -- fluorescence detection -- liquid chromatography; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.202000345
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: A fast, green, sensitive, and accurate analytical method using high‐performance liquid chromatography couple with fluorescence detection was established and validated for the simultaneous determination of amlodipine besylate and celecoxib in their recently approved fixed‐dose combination tablets (1:20). Separation of the two drugs was achieved on C18 reversed‐phase column (Thermo ODS Hypersil, 4.6 × 250 mm, particle size 5 µm) using acetonitrile:potassium phosphate buffer (50 mM; pH 5.5, 60:40 v/v) as a mobile phase at 40°C, which eluted at a rate of 1 mL/min. Detection was carried out with excitation and emission wavelengths of 360 and 446 nm for amlodipine and 265 and 359 nm for celecoxib, respectively. The method was linear over a concentration range of 0.05‐2 and 0.05‐10 µg/mL and limit of detection reached to 0.017 and 0.0167 µg/mL for amlodipine and celecoxib, respectively. The developed method was successfully applied to assess the cited drugs in their newly FDA approved fixed‐dose combination tablet dosage form. Furthermore, the method was found to be sensitive and eco‐friendly green alternative to the reported methods as it was evaluated according to the green analytical procedure index tool guidelines and analytical Eco‐Scale.