Morin decreases acrolein-induced cell injury in normal human hepatocyte cell line LO2. (December 2020)

• Record Type: Journal Article
• Authors: Yin, Zhao; Guo, Hongyang; Jiang, Kaiyu; Ou, Juanying; Wang, Mingfu; Huang, Caihuan; Liu, Fu; Bai, Weibin; Zheng, Jie; Ou, Shiyi
• Is Part Of: Journal of functional foods. Volume 75(2020)
• Publication Date: 2020-12
• Subjects: Acrolein -- Morin -- Apoptotic markers -- Proinflammatory cytokines -- Signaling pathway; ABCB ATP-binding cassette, sub-family B -- ABCC ATP-binding cassette, sub-family C -- AIF apoptosis induced factor -- BAX B-cell lymphoma-associated X -- BCL B-cell lymphoma -- CASP caspase -- COX-2 cyclooxygenase-2 -- Cyto-C cytochrome C -- DMSO dimethylsulfoxide -- EDTA ethylenediaminetetraacetic acid -- ERK extracellular regulated protein kinases -- FBS fetal bovine serum -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- HMOX1 heme oxygenase -- HRP horse radish peroxidase -- IFN interferon -- IL interleukin -- iNOS inducible NO synthase -- JNK c-Jun N-terminal kinase -- KEGG Encyclopedia of Genes and Genomes -- MAPK mitogen-activated protein kinases -- NROB nuclear receptor subfamily -- PARP poly ADP-ribose polymerase -- PBS phosphate-buffered saline -- SAPK Stress-activated protein kinases -- TNF tumor necrosis factor -- UGT1A UDP glucuronosyltransferase; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104234
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Morin decreases acrolein-induced cell damage in the LO2 cell line. It inhibits the expression of apoptotic markers and proinflammatory cytokines. It blocks the ERK/JNK/P38-MAPK signaling pathway. Abstract: Liver is the organ most frequently exposed to acrolein, a priority toxic pollutant. In the present study, whether morin can mitigate acrolein-induced cell damage in normal human hepatocyte cell line LO2 was investigated. Morin remarkably inhibited acrolein-induced apoptosis by inhibiting the expression of apoptotic markers, reducing the formation of proinflammatory cytokines, and blocking the ERK/JNK/P38-MAPK signaling pathway. The addition of equimolar morin decreased acrolein-induced cell apoptosis by 52.4%. The expression levels of apoptotic markers, BAX, Cyto-C, AIF, cleaved caspase-3/9, and PARP, decreased by 16%, 14%, 44%, 56%, 23%, and 45%, respectively. The proinflammatory cytokines, such as tumor necrosis factor α, inducible NO synthase, cyclooxygenase-2, interleukin 1β, and IFN-ϒ were reduced by 39%, 55%, 66%, 72%, and 64%, respectively. The phosphorylation level of ERK1/2, JNK, and P38 MAPK decreased by 10%, 47%, and 26%, respectively. The present findings may provide a way to mitigate acrolein-induced hepatic injury.