Anti-diabetic effects of sea cucumber (Holothuria nobilis) hydrolysates in streptozotocin and high-fat-diet induced diabetic rats via activating the PI3K/Akt pathway. (December 2020)

• Record Type: Journal Article
• Authors: Wang, Tingting; Zheng, Lin; Zhao, Tiantian; Zhang, Qi; Liu, Zhitong; Liu, Xiaoling; Zhao, Mouming
• Is Part Of: Journal of functional foods. Volume 75(2020)
• Publication Date: 2020-12
• Subjects: Sea cucumber -- Bioactive peptide -- Anti-diabetic activity -- Insulin resistance; Akt Serine/threonine-protein kinase -- FBG Fasting blood glucose -- GSK-3β Glycogen synthase kinase 3β -- GLUT4 Glucose transporter 4 -- GHb Glycated hemoglobin -- HDL-C High-density lipoprotein cholesterol -- HOMA-IR Homeostasis model assessment of insulin resistance -- IRS1 Insulin Receptor substrate 1 -- ISI Insulin Sensitivity index -- LDL-C Low-density lipoprotein cholesterol -- MW Molecular weight -- OGTT Oral glucose tolerance test -- STZ Streptozotocin -- SCH Sea cucumber hydrolysates -- TG Triglyceride -- TC Total cholesterol; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104224
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Sea cucumber hydrolysates were extracted from a low-edible-value sea cucumber. Sea cucumber hydrolysates showed hypoglycemic, hypolipidemic and ameliorating insulin resistance effects. Sea cucumber hydrolysates alleviated insulin resistance by triggering PI3K/Akt signaling pathway. Aliphatic amino acids containing peptides might contribute to improve insulin resistance. Abstract: In this study, anti-diabetic activity and potential mechanism of sea cucumber hydrolysates (SCH) were investigated in type II diabetic rats induced by streptozotocin. Results showed that SCH alleviated body weight loss, oral glucose tolerance and insulin resistance in diabetic rats. The fasting blood glucose level was markedly decreased by 40.39% after SCH administration. Moreover, lipid metabolism disorders in diabetic rats were attenuated to near normal after SCH treatment. Mechanism studies showed that the expressions of PI3K, p-Akt, p-GSK-3β and GLUT2/GLUT4 were significantly increased and the expression of p-IRS1 was significantly decreased after SCH treatment in liver and skeletal muscle tissues of diabetic rats. In addition, 25 peptides were screened as potential bioactive peptides by analyzing the intensity of mass spectra combined with PeptideRanker prediction. In summary, these findings suggested that SCH improved insulin resistance in diabetic rats via triggering PI3K/Akt signaling pathway, indicating that SCH may possess anti-diabetic effects.