Wheat peptide protects against ethanol-induced gastric mucosal damage through downregulation of TLR4 and MAPK. (December 2020)

• Record Type: Journal Article
• Authors: Yang, Xian; Yang, Ligang; Pan, Da; Liu, Hechun; Xia, Hui; Wang, Shaokang; Sun, Guiju
• Is Part Of: Journal of functional foods. Volume 75(2020)
• Publication Date: 2020-12
• Subjects: Gastric mucosal damage -- Ethanol -- Wheat peptides -- Anti-inflammatory; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2020.104271
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Excessive alcohol consumption can lead to gastric mucosal damage. Wheat peptides repressed gastric inflammation through the reduction of inflammatory factor. Wheat peptides prevented gastric oxidative stress through the inhibition of lipid peroxides. Wheat peptides down-regulated protein expression levels of TLR4, MAPK and ERK. Ethanol-induced gastric mucosal damage in rats can be prevented by wheat peptides. Abstract: Excessive alcohol consumption can induce gastric mucosal damage. The present work aimed to examine the protective effect of wheat peptides against ethanol-induced gastric mucosal damage in rats. Wheat peptides (WP) were administered daily by gavage for 30 days before the rats were treated with anhydrous ethanol (1 mL). Histological observations were performed, as well as the anti-oxidation effect and anti-inflammatory action. WP increased the superoxide dismutase activity and prostaglandin E-2 (PGE2) levels and decreased the malondialdehyde and interleukin-8 (IL-8) levels. WP suppressed gastric mucosa inflammation through the reduction of TNF-α, MPO, IL-6, and IL-1β. WP repressed the expression of caspase-3, and enhanced the expression of anti-apoptotic protein EGFR. Furthermore, WP down-regulated protein expression levels of TLR4, MAPK and ERK, suggesting that WP have a potential effect to inhibit ethanol-induced inflammatory responses via blocking TLR4/MAPK/ERK signal transduction. The potential mechanism may be associated with enhancement of antioxidation, anti-inflammatory action and anti-apoptosis function.