Detailed characterization of an IncFII plasmid carrying blaOXA-48 from Lebanon. (17th May 2020)

• Record Type: Journal Article
• Authors: Moussa, Jennifer; Panossian, Balig; Nassour, Elie; Salloum, Tamara; Abboud, Edmond; Tokajian, Sima
• Is Part Of: Journal of antimicrobial chemotherapy. Volume 75:Number 9(2020)
• Page Start: 2462
• Page End: 2465
• Publication Date: 2020-05-17
• Subjects: Anti-infective agents -- Periodicals; Chemotherapy -- Periodicals; 615.58
• DOI: 10.1093/jac/dkaa181
• Languages: English
• ISSNs: 0305-7453

Abstract

Abstract: Background: The spread of carbapenem-resistant Enterobacteriaceae is an important challenge and an increasing healthcare problem. OXA-48 is a class D carbapenemase that is usually localized on a conjugative plasmid belonging to the IncL incompatibility group. Methods: In this study, we used a combination of short- and long-read WGS approaches and molecular typing techniques to characterize the genetic environment of the smallest reported 27 029 bp IncFII plasmid carrying bla OXA-48 (pLAU-OXA48). Results: The plasmid recovered from a clinical Escherichia coli isolate was positive for bla OXA-48, which was located within the Tn 6237 composite transposon. Primers targeting junctions between the IncF fragment and Tn 6237 for the rapid identification of pLAU-OXA48-like plasmids were designed. Conclusions: To our knowledge, this is the first report showing the complete sequence of an IncFII plasmid carrying bla OXA-48 within Tn 6237 using hybrid assembly of long- and short-read sequencing.